We authored and co-authored a number of publications in highest impact journals. Skip directly to content. We are now able to establish methods at pulsed X-ray sources that allow us to investigate important proteins and enzymes with static and time-resolved crystallography and provide a tool for a better understanding of structural dynamics, catalysis and the molecular basis of life, specifically photomorphogenesis of myxobacteria. Light-dependent formation of S. When clicking on a Digital Object Identifier DOI number, you will be taken to an external site maintained by the publisher. Prepare Proposals Limited proposal types.
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This is called the ‘diffraction-before-destruction’ principle. Among prokaryotes, myxobacteria are distinguished by a unique multicellular stage in their life cycle in which fruiting bodies are formed.
Back to Previous Page. This project will investigate the role of bacteriophytochromes through integrated studies of myxobacterial genetics, high-throughput RNA sequence analysis, time-resolved spectroscopy, and elucidation of bacteriophytochrome structures by X-ray crystallography.
We authored and co-authored a number of publications in highest impact journals. Any opinions, findings, and conclusions or recommendations expressed in this Report are those of the PI and do not necessarily reflect the views of the National Science Foundation; NSF has not approved or endorsed its content.
Saint Louis Avenue City: Photoreceptors are proteins that enable organisms to perceive and respond to light, an essential environmental signal; however, their physiological role in non-photosynthetic bacteria remains largely unknown. More experiments are underway to push time-resolution to femtoseconds in order to capture the unique intermediates of BphP photocycle.
We are now able to establish methods at pulsed X-ray sources that deaktop us to investigate important proteins and enzymes with static and time-resolved crystallography and provide a tool for a better understanding of structural dynamics, catalysis and the molecular basis of life, specifically photomorphogenesis of myxobacteria.
The students learn how to purify protein and prepare macroscopic and microscopic crystals. Some full text articles may not yet be available without a charge during the embargo administrative interval. Northeastern Illinois University Street: We are currently developing genetic tools to knock out BphP genes in related M.
This project addresses the physiological role of red-light desktoo known as bacteriophytochromes in the non-photosynthetic myxobacteria Stigmatella aurantiaca and Cystobacter fuscus which form fruiting bodies under starvation conditions. Prepare Proposals Limited proposal types. Many living organisms sense and respond to light, primarily through a large family of proteins known as photoreceptors.
Now, microscopically small crystals are sprayed into the ultrashort XFEL pulses. We collaborate nationally and internationally with leaders in the field and provide ample opportunity for our undergraduate and graduate students to lead experiments at the world?
Most importantly, we were able to successfully apply for funding through an NSF EAGER award that enables us in part to continue this exciting research. Interestingly, we were able to demonstrate that S. Emina Stojkovic E-Stojkovic neiu.
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Publications Produced as a Result of this Research Note: This Research in Undergraduate Institutions proposal will provide insights into fundamental, light-induced mechanisms in biological systems by addressing the role of photoreceptors in unique microorganisms, the myxobacteria, which are distinguished by unusual light-controlled morphogenesis of fruiting bodies.
Skip directly to content. We reported results from the last four years at about 20 lectures at workshops and conferences. This project drsktop advance the understanding of microbial light perception and the evolution of photoreceptors, engaging undergraduate and Master’s level students including members of groups under-represented in science in interdisciplinary research in microbial genetics, spectroscopy and structural biology.
In the meantime, we determined the room-temperature structure of S.
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Some links on this page may take you to non-federal websites. Cellular Dynamics and Function Program Officer: Starvation-induced fruiting bodies house desiccation-resistant spores that can germinate into a vegetative cell upon nutrient availability. We successfully determined the crystal structures of two BphPs from S. The PI teaches a course in Biochemistry, which not only covers crystallography but also all relevant techniques required to perform a successful crystallographic experiment at the synchrotron.
The intense X-ray beam destroys the crystals, yet a diffraction pattern is recorded before the crystals disintegrate. When clicking on a Digital Object Identifier DOI number, you will be taken to an external site maintained by the publisher. Petra Edlund, Emina A.